5.APAAP法 APAAP——碱性磷酸酶抗碱性磷酸酶(Alkaline phosphatase antialkaline phosphatase, APAAP)法是Mason和Moir(1983)等在PAP法的基础上,用AKP替代HRP而建立的一种方法,都属于...
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方法采用碱性磷酸酶抗碱性磷酸酶法(APAAP法)检测48例急性荨麻疹患者和20例健康对照者外周血CD 3 +,CD 4 +,CD 8 + T淋巴细胞。
Methods the peripheral blood t lymphocytes and their subsets in 48 patients with acute urticaria were detected by APAAP and compared with 20 healthy control.
方法:用碱性磷酸酶抗碱性磷酸酶法检测再障患者骨髓中T淋巴细胞表型的变化,并用半固体培养法检测再障患者骨髓及去T细胞后骨髓单个核细胞CFU-GM集落形成情况。
Methods: Phenotype of T lymphocytes in bone marrow and CFU GM yields of bone marrow mononuclear cells with or without T cells were assayed with APAAP and semi solid culture method, respectively.
方法用碱性磷酸酶-抗碱性磷酸酶(APAAP)免疫酶标和DNA末端原位标记(ISEL)双重染色方法检测38例急性白血病诱导化疗前后骨髓涂片中细胞凋亡程度及细胞类型,初治组17例与2 1例经治病例对照。
Methods A double-stained way of APAAP and in situ DNA end labeling (ISEL) were used to detect apoptosis characteristics in 38 cases of acute leukemia, including 17 untreated and 21 treated patients.
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