另外获得了‘萨蔓莎’和‘金斯莱克’的抗性愈伤组织。
The Kan-resistant callus of 'Samantha' and 'Jimthyianky' are acquired.
以CC培养基作为共培养后抗性愈伤筛选的基本培养基有利于抗性愈伤组织的筛选培养。
The CC medium was an efficient selectable medium for screening hygromycin resistant calli after co cultivation.
最佳处理条件下转化683枚八棱海棠叶片,共得到138个抗性愈伤组织和15株抗性苗,转化率为2.2%。
Total 683 leaves were transferred under the optimum treatment condition, then 138 resistant callus and 15 resistant shoots were obtained (transformation rate 2.2%).
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