目的探讨在不同的免疫学检测方法中，与单链抗体相连的亲和标记肽对单链抗体与抗原结合活性的影响。
Objective to investigate the affects of affinity tag on binding activity of single chain variable fragment (ScFv) to antigen using different immunological assays.
为了降低人抗鼠抗体反应，获得满意的免疫原性，将模拟人卵巢癌抗原的抗独特型单链抗体人源化。
In order to reduce the human anti mouse antibody response and obtain optimal antigenicity, anti idiotype single chain which mimicking ovarian cancer antigen has been humanized.
结论利用噬菌体抗体库技术获得了人源性抗角蛋白单链抗体，为临床应用研究提供了具有更广阔应用前景的人源小分子抗体。
Conclusion Human anti-keratin scFv is obtained from a large phage antibody library, which will benefit to the application of anti-keratin antibody to clinical research.
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