抑制性消减杂交技术(suppression subtractive hybridization,SSH)是以抑制性PCR为基础的基因差异显示克隆技术,该技术是一种快速有效分离差异表达基因的方法,与其它差异...
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用抑制性消减杂交技术 suppression subtractive hybridization
目的应用抑制性消减杂交技术构建人肾癌差异表达基因文库并进行差异表达基因筛选。
Objective To screen the genes differentially expressed in human renal cell carcinoma (RCC) cell lines using suppression subtractive hybridization (SSH).
结论DADS能诱导人白血病HL 6 0细胞分化，并引起相关的基因发生改变，而抑制性消减杂交技术能有效地分离差异表达的基因。
CONCLUSION DADS can induce human leukemia HL 60 cells differentiation, and suppression subtractive hybridization (SSH) technique is an effective method to isolate those differentially expressed genes.
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