...摘要: 抑制性消减杂交（SSH）是以抑制性PCR为基础结合标准化与消减杂交技术、建立的一项筛选与分离未知差异表达基因的新技术，该技术具有快速、简便、灵敏、特...
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...抑制性消减杂交（SSH） 服务简介 抑制性消减杂交（Suppression Subtractive Hybridization，SSH）是利用PCR 反应链内退火优先于链间退火的特点和分子杂交二级动力学原理，经过体系不断优化建立起来的快捷、有效地差异基因筛...
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抑制性消减杂交技术 suppression subtractive hybridization
用抑制性消减杂交 suppression subtractive hybridization ; SSH
应用抑制性消减杂交 suppression subtractive hybridization ; suppressedsubtractivehybridization
采用抑制性消减杂交 suppression subtractive hybridization
用抑制性消减杂交技术 suppression subtractive hybridization
利用抑制性消减杂交 suppression subtractive hybridization
然后用抑制性消减杂交 Suppression Subtractive Hybridization
和抑制性消减杂交 suppression subtractive hybridization
抑制性消减杂交方法 suppression subtractive hybridization
抑制性消减杂交是一种简便易行、 特异性高的高效分离差异表达基因的方法。
Suppression subtractive hybridization(SSH), which is feasible and highly specific, is one of the most efficient methods for cloning differentially expressed genes.
目的应用抑制性消减杂交技术构建人肾癌差异表达基因文库并进行差异表达基因筛选。
Objective To screen the genes differentially expressed in human renal cell carcinoma (RCC) cell lines using suppression subtractive hybridization (SSH).
结论DADS能诱导人白血病HL 6 0细胞分化，并引起相关的基因发生改变，而抑制性消减杂交技术能有效地分离差异表达的基因。
CONCLUSION DADS can induce human leukemia HL 60 cells differentiation, and suppression subtractive hybridization (SSH) technique is an effective method to isolate those differentially expressed genes.
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