本文综述了DNA序列分析、P CR限制性片段长度多态性技术和环介导等温扩增技术在带绦虫虫种鉴别中的应用。
This paper summarizes the application of molecular biological techniques in the identification of Taenia, such as analysis of DNA sequence, PCR-RFLP and LAMP.
采用聚合酶链式反应(PCR)技术对来自北京等地区的173份PCV2阳性样本的全长ORF2基因进行了扩增,并对扩增产物进行了限制性片段长度多态性(RFLP)分析。
Whole ORF2 genes of 173 positive clinical PCV2 samples from Beijing and other areas were amplified by PCR and followed by restriction fragment length polymorphism (RFLP) analysis.
运用限制性片段长度多态性分析对PCR扩增产物进行鉴定。
PCR product was confirmed by restriction fragment length polymorphism analysis.
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