方法从胚胎大鼠的大脑皮质、海马、纹状体等组织分离神经干细胞，用无血清培养技术在体外进行培养、扩增、传代和诱导分化。

The advantage of serum free and clone culturing technology was performed to isolate, culture, passage and induce neural stem cells from embryonic rat cortex, hippocampus and striatum.

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另外研究血管内皮生长因子(VEGF)和传代培养对细胞分化、扩增动力学和细胞凋亡的影响。

In addition, we tested the effect of vessel endothelial growth factor (VEGF) and passage on cell differentiation, expansion kinetics and apoptosis.

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结果原代的小鼠培养骨髓基质细胞能很好的维持精原干细胞的非分化性扩增。

The feeder layers of bone marrow stromal cells allowed spermatogonial stem cells to proliferate well and remain undifferentiated.

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So, reproduction, proliferation, cell growth happens and then those cells become more differentiated.

所以 繁殖 扩增 细胞开始生长,然后这些细胞开始分化

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It goes - going through this process of amplifying divisions, every division increasing the number of cells by a factor of two and these cells becoming more differentiated around the way.

它经历了一系列扩增性的分裂过程,每次分化,细胞数目都翻一番,而在此过程中细胞分化得更成熟

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