目的：建立一种高效、快速的大肠杆菌感受态细胞制备及质粒转化的方法。

Objective: To establish an efficient and rapid method for the preparation and transformation of competent cells from Escherichia coli.

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目的研究氯化铷法制备感受态细胞的转化效率和不同条件对其的影响。方法采用氯化铷法制备感受态细胞，用不同的质粒进行转化。

Objective to investigate the transformation efficiency and affecting factors in the preparation of competent E. coli cells by using rubidium chloride (RbCl) method.

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