细菌感受态的制备和转化；
目的：建立一种高效、快速的大肠杆菌感受态细胞制备及质粒转化的方法。
Objective: To establish an efficient and rapid method for the preparation and transformation of competent cells from Escherichia coli.
感受态形成过程中，细胞可能发生了一系列的生理变化。
The physiologic change could take place in the process of forming competent cells.
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