正交设计可提高试验效率及准确性，是愈伤组织诱导培养基、分化培养基筛选优化的好方法；
Orthogonal design improved experimental efficiency and precision, which was a good way for selection and optimization of callus induction medium and differentiation medium.
对于愈伤组织的再分化，不仅需要细胞分裂素，而且诱导培养基中生长素浓度的影响也是显著的。
With regard to the callus redifferentiation, not only kinetin is needed, but also the effect of auxin concentration of the induction medium is also remarkable.
采用正交设计方法，研究了NAA、6-BA、MS培养基对明党参叶片与叶柄愈伤组织诱导的影响。
Effects of NAA, 6-BA, MS culture medium on leaf and petiole callus induction of Changium smyrnioides Wolff were studied by orthogonal design.
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