The growth of suspension cell lines was affected by sucrose . nitrogen nutrition , 2 , 4-D concenration - initial cell density and sub-cultured period Low level of 2, 4-D（0.1 1.0mg/L）.
3．培养基中氮索营养、2,4-D浓度、蔗糖含量以及悬浮细胞系的起始接种量、继代周期等培养条件等对悬浮细胞的生长有重要影响。
参考来源 - 草莓（Fragaria ananassa Duch.）悬浮细胞培养与植株再生的研究In addition, the cells of the Acacia melanoxylon were small, of strong embryogenic characteristics and fine dispersion, which were easy to establish the cell suspension system.
黑木相思细胞小，胚性强，分散性好，最容易建立起悬浮细胞系。
参考来源 - 相思树的细胞培养及体胚发生研究We established 6 Arabidopsis thaliana suspension cell lines and 1 callus line of different genotypes, which gave a useful material for the following experiments.
本研究中成功建立了拟南芥不同基因型的共6个悬浮细胞系，1个愈伤组织系，可作为下一步研究的基础。
参考来源 - 拟南芥悬浮细胞超低温保存及脱落酸在胁迫信号转导途径中的作用研究·2,447,543篇论文数据，部分数据来源于NoteExpress
建立了脱菌的悬浮细胞系；
The suspended cell lines of bacteria-free tissue were set up.
实验中首先建立了稳定的水稻悬浮细胞系。
本文分析了从大麦成熟胚培养快速建立胚性悬浮细胞系的两个关键步骤。
The present paper analysed two key procedures for rapid establishment of embryogenic cell suspension cultures derived from barley mature embryo.
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