结论自体骨髓单个核细胞移植合用中药可以改善下肢缺血患者的临床症状及体征。

Conclusion the integrated therapy of autologous bone marrow mononuclear cell transplantation and TCM drugs can relieve the clinical symptoms and signs in the patients of lower limb ischemia.

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方法：用碱性磷酸酶抗碱性磷酸酶法检测再障患者骨髓中T淋巴细胞表型的变化，并用半固体培养法检测再障患者骨髓及去T细胞后骨髓单个核细胞CFU-GM集落形成情况。

Methods: Phenotype of T lymphocytes in bone marrow and CFU GM yields of bone marrow mononuclear cells with or without T cells were assayed with APAAP and semi solid culture method, respectively.

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在2 9例供血员的外周血单个核细胞（MNC）均未检测到WT1基因表达，但在2 1例非恶性疾病患者骨髓MNC中有8例呈WT1基因阳性（38.1% ）。

None was WT1 positive in peripheral blood mononuclear cells (MNC) from 29 blood donors, while bone marrow MNCs from eight of 21 cases (38.1%) of nonmalignant patient WT1 gene expression were found.

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