异硫氰酸胍是一种化学物质,分子式是CH5N3·HSCN。
中文名称:异硫氰酸胍 英文名称:Guanidine Thiocyanate 分子式:CH5N3HSCN 分子量:118.16 CAS:593-84-0 外观:白色结晶 干燥失重:≤0.5% 含量(滴定法):≥
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异硫氰酸胍(Guanidinium isothiocyanate): 为目前一种最有效的RNA酶抑制剂。它不但具有败坏 细胞结构、核酸从核蛋白质中解离,同时也使RNA酶失活。
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A.2.3 钩端螺旋体DNA 提取(Boom法) A.2.3.1 试剂配制 裂解液(L6):GuSCN(异硫氰酸胍)120g、0.1M Tris-HCl pH6.4 100mL、0.2M EDTA 22mL,混合后用NaOH 调pH 至8.0,加Triton X-100 2.6g; Diatom(硅藻):三蒸水50mL...
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异硫氰酸胍/硫氰酸胍 Guandine thiocyanate
异硫氰酸胍盐 Guanidine thiocyanate ; GITC
异硫氰酸胍-酚氯仿法 guanidine isothiocyanate - phenol chloroform
采用异硫氰酸胍 Guanidine Thiocyanate
异氰硫酸胍 GdnSDN
The result showed that the total RNAs obtained by Improved Guanidine Thiocyanate method were highly pure and undegraded.
结果表明:改进异硫氰酸胍法获得的总RNA纯度高、完整性好。
参考来源 - 梨果实cDNA文库的构建·2,447,543篇论文数据,部分数据来源于NoteExpress
以玛咖无菌苗叶片为材料,通过改良的异硫氰酸胍法提取其总rna。
By using the improved isothiocyanate method, total RNAs were extracted from leaves of sterile Maca plantlet.
用异硫氰酸胍法提取鱼油、加入牛肉DNA的鱼油、加入山羊肉DNA的鱼油和牛油中的DNA。
The total DNAs of fish oil, fish oil added with bovine DNA, fish oil added with goat DNA and butter were extracted by guanidine isothiocyanate method.
该方法与异硫氰酸胍法或冷酚法等相比具有更简便、得到的棉花组织总rna完整性好和纯度高等优点。
The method could produce cotton total RNA with higher purity and integrality in a shorter time from different tissues of cotton than other conventional methods.
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