进化酶基因的DNA测序表明4 186含两个有义氨基酸置换：k16 7r和S374C，二者既不位于底物结合位点，也不位于酶的金属离子结合位点。
DNA sequence revealed that 4-186 contains two amino acid substitutions, K167R and S374C, both of which locate neither the substrate - binding sites nor the metal - binding sites of EAP.
酶的催化中心是由一组氨基酸组成，其三维结构形成活性位点，允许它高效地结合或修饰底物。
The catalytic center of an enzyme is composed of a set of amino acids whose three-dimensional arrangement forms the "active-site" that allows them to efficiently bind and modify the substrate.
当产物积累超出最佳数量时，它将与酶的一个氨酰基位点(变构位点)结合改变其形状，使其不能长时间与底物发生作用。
When the product accumulates beyond an optimal amount it binds to a site (ALLOSTERIC site) on the enzyme, changing the shape so that it can no longer react with its substrate.
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