然后在水迷宫训练后的第1、7、14天，应用免疫组化染色检测齿状回神经干细胞的增殖及存活情况。
The proliferation and survival of neural stem cells were detected with immunohistochemical staining at 1 7 and 14 days after training.
方法：应用免疫组化染色s - P法，检测88例乳腺癌组织石蜡切片中MMP9及CD 147的表达情况。
Methods: Expression of MMP9 and CD147 protein in 88 patients with breast cancer was measured by immunohistochemistry (S-P) method.
方法在建立大鼠实验性牙周炎的基础上，应用免疫组化染色观察er、PR在牙龈组织的表达，并与正常对照组进行比较研究。
Methods Based on experimental periodontitis of rat, the expression of er, pr were observed by immunohistochemical method in periodontitis tissue and compared with normal group.
应用推荐