...期 【摘要】： 目的在293T和Mel细胞中研究RNA干扰(RNAinterference,RNAi)对绿色荧光蛋白(enhancedgreenfluorescentprotein,eGFP)表达的沉默作用。
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通过转化绿色荧光蛋白基因质粒，对阿特拉津降解基因工程菌进行标记。
The atrazine-degrading genetically engineered microorganism(GEM) was labeled by transforming a plasmid containing green fluorescent protein(GFP) gene.
目的构建携带报告基因增强型绿色荧光蛋白（EGFP）的反转录病毒载体，并且探讨病毒载体对SK-N-SH神经母细胞瘤细胞株的感染效率。
Objective To construct the retroviral(RV) vector with report gene enhanced green fluorescent protein(EGFP) and to explore the gene transfection efficiency of RV on SK-N-SH neuroblastoma cells.
目的通过对人退变髓核细胞的原代培养，了解其生物学特性，并将绿色荧光蛋白基因转染至细胞内。
Objective Human degenerative nucleus pulposus cells were cultured in monolayer, the biological action of the cells was observed.
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