...中心 关键词】 寡核苷酸的固定；基因芯片技术；丙烯酸类聚合物；共价交联 [gap=891]Keywords: oligonucleotides immobilization; DNA microarray; acrylic acidcoacrylamide copolymer; covalent linkage ..
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寡核苷酸固定过程中，末端氨基修饰没有明显的特异性，但是可以提高被固定探针的杂交容量。
During oligonucleotide immobilization, terminal amino modification has no obvious specificity, but it can enhance the hybridization capacity of immobilized oligonucleotides.
紫外交联对大片段DNA固定率影响显著，但对氨基修饰的寡核苷酸影响不显著；
Ultraviolet crosslinking greatly affected the attachment rate of a large fragment of DNA, but did not noticeably affect the attachment rate of the amino-modified oligonucleotides.
本文介绍了寡核苷酸适配子作为生物识别元件在生物传感器中的应用以及作为色谱固定相的应用。
In this review, were discussed the applications of aptamers as biocomponents in biosensors, and the applications of aptamers as chromatographic stationary phases.
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