...实时定量聚合酶链反应; 牙周临床指标 [gap=1976]Key words] fixed orthodontic treatment; Porphyromonas gingivalis; real-time quantitative polymerase chain reaction; clinical periodontal index ..
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...外周血单个核细胞,磁珠分离CD4+T细胞、CD8+T细胞;(2)试剂盒提取RNA和DNA;(3)实时定量聚合酶链反应(quantitative real-time PCR)检测CD40LG的mRNA的表达。(4)
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...ON)》-2002 校坤, 吴帆, 期刊- 核心期刊 ISSN : 1000-9965(2002)05-0116-07 实时定量聚合酶链反应(real-time quantitative PCR,RQ-PCR)是综合PCR、分子杂交和酶动力学的一种新技术,它根据荧光共振能量转移(fluorescence re..
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...后1,2,3,5,7,10d(各6只眼)伊文氏兰示踪法检测新生血管渗透率,实时定量聚合酶链反应(Real-time polymerase chain reaction,Real-time PCR)法检测角膜组织中VEGF-RNA的表达。以正常大鼠角膜(6只眼)作为对照。
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目的探讨实时荧光定量聚合酶链反应(FQ - PCR)在检测外周血清及单核细胞中hcv RNA含量的临床应用价值。
Objective To investigate the clinical application of real time fluorescence quantitive Polymerase Chain Reaction (FQ-PCR) in detection of HCV RNA in serum and peripheral blood monocular cells (PBMC).
经实时荧光定量逆转录聚合酶链反应验证了基因芯片准确可靠。
The results of realtime RTPCR proved the accuracy of the gene chip.
方法采用实时荧光定量聚合酶链反应和免疫组化染色法检测96例食管癌患者的食管癌组织和癌旁正常组织Caspase-8表达。
Methods We quantified Caspase-8 expression levels in 96 esophageal cancer issues and their matched normal esophagus tissues by using real-time PCR assay and immunohistochemistry staining.
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