实时定量 (real-time quantitative )是指在指数扩增期间通过连续监测荧光信号强弱的变化来即时测定特异性产物的量，并据此推断目的基因的初始量，不需要取出产物进行...
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文章详细信息 关键词： 实时定量;PCR;小麦 [gap=188]Keywords: Real-time quantification;PCR;Wheat
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实时定量PCR real time PCR ; Real-time quantitative PCR ; Real-time PCR ; EvaGreen dye
实时定量聚合酶链反应 real-time quantitative polymerase chain reaction ; quantitative real-time PCR ; real-time quantitative PCR ; real-time Polymerase Chain Reaction
实时定量RT-PCR Quantitative Real-Time RT-PCR ; real time rt-pcr ; real time quantitative RT-PCR
实时定量PCR试剂盒 qPCR
荧光实时定量PCR Real time quantitative PCR ; quantitative real time PCR ; real-time pcr ; Florescent real-time quantitative RT-PCR
实时定量 PCR real-time quantitative PCR
实时定量RT Quantitative Real-Time RT-PCR
半巢式实时定量pcr semi-nested qrt-pcr
实时定量多聚酶链反应 real-time polymerase chain reaction ; REAL-TIME PCR ; real-time quantitative polymerase chain reaction
Real-time quantitative PCR was performed with three primer pairs,respectively,for fecal samples from 14 healthy individuals.
用3对引物分别对14个健康人的粪便样品进行实时定量PCR。
参考来源 - 检测肠道细菌Feacalibacterium prausnitzii的三对PCR引物的特异性比较The reliability of the data obtained from the microarray was verified by performing quantitative real-time PCR on 12 representative genes.
挑选12个基因应用实时定量PCR对芯片数据进行验证,结果与芯片的数据吻合。
参考来源 - 猪链球菌毒力因子与免疫原性蛋白的筛选及斑马鱼模型转录组研究·2,447,543篇论文数据，部分数据来源于NoteExpress
进行实时定量PCR反应，建立标准曲线；
Real-time PCR reaction; Amplification curve output; Ct data obtain;
用实时定量pcr检测标本线粒体dna的拷贝数。
Moreover, to detect the mitochondria DNA copy Numbers of sample with real-time PCR.
免疫荧光法和实时定量PCR有较好的一致性（92.3%）。
The correspondence of the HCMV pp65 test and quantitative PCR was 92.3%.
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