方法提取患者脑脊液和血清中的病毒rna，用肠道病毒特异性引物进行套式rt - PCR扩增，检测特异性基因片段。

Methods RNA of virus was obtained from samples of cerebrospinal fluid and serum and then gained with specific primer of Enterovirus through RT-PCR so as to detect specific gene fragment.

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该研究通过设计合成hla基因序列特异性引物，建立了HLA - DR基因分型的套式扩增和直接扩增pcr - SSP技术，并在骨髓移植配型中进行了应用。

In this study, a nested PCR SSP and a direct amplification PCR SSP protocols for HLA DR genotyping were developed and were used in the selection of matched donor for sibling BMT.

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