方法取大鼠胚胎分离成纤维细胞，分别采用细胞悬液法和胰酶消化植块培养法对其进行培养。
Methods Separate the embryonic fibroblast of mice and culture with the method of suspension culture and method of trypsinization.
通过比较LIF和大鼠心肌条件液在小鼠es细胞分离培养过程中的差异，从而选择较为合适的培养液用于小鼠es细胞的分离培养与深入研究。
By comparing the difference between LIF media conditioned and rat cardiomyocyte media conditioned during isolation and culture of mouse ES cell, suitable media is selected in the future.
方法：采用胶原酶灌注法及剪碎消化法分离大鼠胎肝干细胞，并用含10%优等胎牛血清的H - DMEM培养液培养。
Methods Collagenase perfusion method and mechanical cutting method were used to isolate HSCs from rat fetal liver which were then cultivated by H-DMEM containing 10% fetal bovine serum.
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