方法用健康大鼠进行急性毒性试验；
Methods Acute cutaneous toxic experiment was performed on the skin of healthy rats;
方法进行了常规的灭螺试验和鱼类急性毒性试验以及大鼠急性经口毒性试验。
Methods The normal test of killing snail and acute toxicity test to fish and mouse were carried out in laboratory.
方法采用大鼠足跖肿胀试验、大鼠毛细血管通透性实验、小鼠扭体法镇痛试验和小鼠急性毒性试验。
Methods rat pedal swelling experiment, rat capillary permeability experiment, mouse acetic-acid-induced twisting experiment and mouse acute toxicity experiment were carried out in this study.
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