...;;细胞相对活性;;外源蛋白表达;;人β干扰素 [gap=975]Keywords: Pichia pastoris;;cell viability;;foreign protein expression;;human IFNβ-HSA fusion protein ...
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毕赤酵母表达系统是目前最为成功的外源蛋白表达系统之一。
The Pichia pastoris is one of the most successful foreign protein expression system until now.
结果经SDS PAGE和薄层扫描分析表明,外源蛋白表达量占菌体裂解蛋白总量的6 . 4 %。
Results SDS PAGE and thin layer scanning showed that the expressed product contained about 6.4% of total somatic protein.
要在一种宿主表达系统中成功表达外源蛋白并获得较高产量,必须要较为全面地了解影响其表达的诸多因素。
Numerous factors that influence heterologous protein production in a certain host system must be observed to gain successful gene expression and even with high yields.
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