结论：可通过四环素抗性筛选系统筛选外源基因的高效表达克隆。
Conclussion: We could screen high expressed clone of heterologous gene using tetracycline resistance screening system.
PCR分子检测与转化的标记基因和外源目的基因抗性三者极有规律性。
The results of PCR molecular detection, marker genes and foreign target-gene with insect-resistance were quite regular.
P CR检测结果表明，37株卡那霉素抗性植株中30株为阳性，说明vp7外源基因已整合到受体植物基因组中。
PCR detection results showed that 30 plants showed positive in 37 kanamycin resistance plants. Its indicated that the foreign gene VP7 had already integrated into genome of receptor plants.
应用推荐