It was easy to obtain the gametophyte material that was plentiful collected directly outside and be used to do the explant culture. But the critical step of this method was to sterilize the material.
直接将野外采回的大量茎叶体进行外植体培养,材料获得容易,但材料的消毒十分关键。
参考来源 - 几种藓类植物配子体再生体系的建立·2,447,543篇论文数据，部分数据来源于NoteExpress
方法：将丹参外植体培养在含不同激素配比的诱导培养基上，并对其愈伤组织中所含次生代谢产物进行分析。
Method:Study the development of allochtonic formations in the callus from explants of Salvia miltiorrhiza cultured on hormones of different content ratios in the medium.
以佛手山药块茎、叶片、茎段为外植体，探讨了其组织培养技术。
Tissue culture of Dioscorea opposita "foshou" was studied with explants such as tubers, leaves, stem segments.
以东方百合“索邦”的鳞片为外植体，进行组织培养技术研究。
The Oriental lily squamas of Sorbonne were used as explants for tissue culture.
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