结果经酶切和测序鉴定表明所构建的重组表达质粒中含有TSO45 - 4b基因。
Results: the constructed recombinant plasmid contained the sequence of TSO45-4B gene that was identified by endonuclease digestion and sequence analysis.
同时在连续传代40代后每隔5代提取重组病毒全基因组进行酶切鉴定，发现其酶切位点保持不变。
To evaluate its heredity stability, the recombinant virus genome was extracted and digested by Hindlll every 5 generations, and 40 generations later, its enzyme digestion sites almost keep stable.
经pcr鉴定、酶切鉴定和测序说明所克隆的两种基因是正确的。
Both genes were correctly cloned and identified by PCR, restriction enzyme digestion and sequencing.
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