在现代分子生物学技术研究中,常常需要对已知位点的侧翼序列进行分析或克隆,以研究基因的遗传表达调控。
In modern research of molecular biology, we usually need to analyse or clone these flanking sequence in given sites, so as to study gene expression and control.
并将外源基因alb亚克隆入该调控序列内,转染c 127细胞,经免疫组化法检测其表达情况。
Subclone the exogenous gene ALB into the regulating sequence for transfection of C127 cells.
在真核细胞,尽管一些转录活性基因和转录沉默基因的调控序列位置常常非常接近,它们的表达却并不互相影响。
In the eukaryotic cells, despite how close the regulation elements of active and inactive genes are frequently, they dot not affect each other expression level.
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