第二部分：构建表达hNT-3基因的慢病毒载体(lentiviral vector-hNT-3，LV/hNT-3)，实验组根据最佳MOI用LV/hNT-3转染NSCs，对照组NSCs不转染病毒。
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结论成功构建了带有大鼠bmp - 7基因的慢病毒载体，并实现其在HSC - T6的稳定高表达。
Conclusion Lentiviral vector carrying BMP-7 gene has been successfully constructed and maintains high expression in HSC-T6 cells.
目的：探讨慢病毒载体在白血病细胞中的基因转导效率，为白血病基因治疗提供关键依据。
Objective to investigate the gene transduction efficiency of lentiviral vector in leukemia cells to provide key basis for leukemia gene therapy.
目的构建表达人类白细胞抗原- E (HLA - E)基因慢病毒载体，探讨慢病毒介导HLA - E基因在肿瘤免疫中的意义。
Objective to construct the lentiviral expression vector of HLA-E gene and investigate its significance for further study on tumor immunity.
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