近年来植物基因克隆技术已经取得了很大进展。

Great achievements have been made in the technology for plant gene isolation.

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因而，原生质体制备和再生技术是禾长蠕孢菌转化与基因克隆技术的基础。

So it is a key step to establish an effective system of preparation and regeneration of HGE protoplasts.

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因此，我们在扪心自问为什么对克隆技术如此着迷之前，不得不首先审视自己的心灵，问一问：究竟是什么东西使得我们中的许多人对于尝试复制与自身基因完全等同的孪生兄弟那么不安？

So before we can ask why we are so fascinated by cloning, we have to examine our souls and ask, What exactly so bothers many of us about trying to make an exact copy of our genetic selves?

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Another example of using this technique is to produce therapeutic proteins from cloned DNA and I'm going to describe this one.

另一个运用到此技术的例子就是,从克隆的基因中制造治疗性的蛋白质,我会具体说这个例子

耶鲁公开课 - 生物医学工程探索课程节选