在人外周血淋巴细胞(human peripheral blood lymphocyte,hPBLC)鼠移植模型中,多数人认为引起异种GVHD的主要原因为供者T(CD3+)细胞[2、3、5、6]。
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目的与方法:利用碱性单细胞凝胶电泳(SCGE)技术在体外检测硫芥对人外周血淋巴细胞的损伤情况。
Purpose and Methods: Using the alkaline single cell gel electrophoresis technique (SCGE) detect the DNA damage of lymphocytes in human peripheral blood, which were exposed to Sulfur mustard in vitro.
在一定剂量范围内,人外周血淋巴细胞克隆效率与照射剂量呈负相关,HPRT基因突变频率与照射剂量呈正相关。
In the radiation dose range used, the colony efficiency of the lymphocytes was negatively correlated to the radiation dose, while the HPRT mutation frequency is positively correlated.
本论文在本实验室前期研究基础上,初步建立起了利用彗星试验研究中药单体对人外周血淋巴细胞DNA的损伤与保护作用的方法体系。
In addition, a new method was set up to detect the DNA damage and protection effect of herb compounds on the human peripheral lymphocyte.
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