用DNA梯状凝胶电泳法(DNA ladder electrophoresis)和流式细胞检测技术(flow cy-tometry,FCM)分别检测HL-60细胞的凋亡率。
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用间接免疫荧光染色结合流式细胞技术检测细胞表面DR4和DR5分子表达；
The expression of cell surface-bound DR4 and DR5 were determined by indirect fluorescence staining and flow cytometry analysis.
采用双标记流式细胞分析技术，分别检测各组小鼠脾脏及肠系膜淋巴结（MLN）CD80 M和CD86 M的表达。
The number of CD80/CD86 M of spleen and mesenteric lymph node(MLN) were determined by double-label FCM method.
并用TUNEL技术和流式细胞仪检测了相关淋巴细胞的凋亡。
TUNEL and flow cytometry were used to detect apoptotic cells.
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