方法:(1)采用腹腔注射胰蛋白酶法,分离培养SD大鼠原代腹膜间皮细胞,经倒置相差显微镜和免疫组织化学染色(Immunohistochemistry,IHC)鉴定;(2)常规传代,取第二代腹膜间皮细胞用于实验,培养的细胞静止24h后,随机分为正常对照组和TG...
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学和免疫组织化学染色肝脏样本，从所有的动物被处理的光镜。
Histological and immunohistochemical stains Liver samples from all animals were processed for light microscopy.
方法采用常规组织学染色和免疫组织化学染色方法对家兔脊神经节内肥大细胞和P物质免疫阳性反应进行观察。
Methods Normal tissue stain and immunohistochemical stain were used to observe the mast cell and substance P immunoreactivity in dorsal root ganglia.
用多巴(DOPA)染色和免疫组织化学对细胞鉴定。
The RPE cells were identified by DOPA staining and immunohistochemistry.
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