方法采用急性粒细胞白血病细胞可溶性蛋白抗原致敏体外培养的DC，并通过MTT法测定CTL活性。

Methods The dendritic cells(DCs) were cultured in vitro and induced by SPA. The activity of CTLs was detected by MTT.

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本文报告了采用SDS—PAGE垂直板电泳技术对脆弱类杆菌及相关菌株超声波粉碎的可溶性蛋白抗原进行了电泳谱分析。

By using SDS-PAGE technique the soluble protein antigen of anaerobic bacteroides fragilis were studied. The results show three kinds of protein zone among the strains species.

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结论只表达目的基因的抗原结构域，可以缩短表达片段的长度，有利于获得可溶性目的蛋白，并且具有良好的血清学反应的特异性。

The antigen regions of the aimed gene were selected to be expressed, which could benefit the expression of soluble fusion proteins, and the speciality of serological reaction is satisfied.

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