方法采用标准微电极及双微电极电压钳技术。
METHODS standard microelectrode and two microelectrodes voltage clamp techniques were used.
方法应用双电极电压钳技术分别在带滤泡膜及去滤泡膜后注入RNA并经孵育的卵母细胞记录膜电流。
Methods Dual-electrode voltage clamp technique was used to record membrane currents from follicular oocytes, and defolliculated oocytes that had been injected with alien RNA and incubated.
方法：在去除滤泡膜的非洲爪蟾卵母细胞上，建立双电极电压钳记录技术，并使用该技术观察爪蟾卵母细胞表达的内源性递质门控性和电压门控性离子通道电流。
Methods: the two-electrode voltage clamp recording technique was used to observe the ligand-gated and voltage-gated ion channel currents expressed in Xenopus laevis oocytes without follicles.
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