双抗体夹心法，是将含有已知抗体的抗血清吸附在微量滴定板上的小孔里，洗涤一次；加待测抗原，如两者是特异的，则发生结合，然后把多余抗体洗除；加入与待测抗原呈特异反应的酶联抗体，使形成“夹心”；加入该酶的底物，若看到有色的酶解产物产生，说明在孔壁上存在相应的抗原。
双抗体夹心法（double antibody sandwich method,sandwich ELISA）是一种测定待测标本中是否含抗原的方法,步骤为：1,将含已知抗体的抗血清吸附在微量滴定板（microtiter plate ,聚...
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...质芯片,双抗体夹心法,光学信号灰度值 [gap=1530]Key words】immun-gold staining; HCG; protein chip; double-antibody sandwich; intension of the staining IC spots ...
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关键词：儿童 ；CK-MB ；酶免疫抑制法 ；双抗体夹心法 [gap=1503]Key words: Child; CK-MB; Enzyme Inhibition Immunoassy; Double Antibody Sandwich Enzyme-Link Immunoassay
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Objective: to establish a sensitive and special method of double mono-cloned antibody for examining the circular immune complex (CIC) in blood, and to further probe the relation between CIC of chlamydia pneumoniae (Cpn) and the coronary heart disease.
目的：建立一种敏感而且特异的双抗体夹心法检测血液中的循环免疫复合物(CIC)，并讨论血液中肺炎衣原体(Cpn)CIC与冠心病的关系。
参考来源 - 双抗体夹心法检测肺炎衣原体循环免疫复合物的方法学建立·2,447,543篇论文数据，部分数据来源于NoteExpress
采用酶法及酶联免疫双抗体夹心法测定血浆脂蛋白的浓度。
The concentrations of plasma lipoproteins were determined with enzyme and enzyme linked immunoassay double antibody methods.
用免疫放射双抗体夹心法测定，标记抗体能保持良好的免疫活性。
The labelled antibody showed good immunoreactivity, when it was measured by IRMA-bi-antibody sandwich method.
结果：该双抗体夹心法，底物显色反应清楚肉眼即可作出判定，非特异干扰极小。
Results: The colored reaction of sandwich assay is very clear and easily judged by naked eyes.
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