目的制备多种抗猪鼻支原体的单克隆抗体，建立双抗体夹心ELISA方法用于该病原体的检测。

Objective To prepare monoclonal antibodies(McAbs) of Mycoplasma hyorhinis and establish an antibody-sandwich ELISA method for the detection of the organism.

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方法：采用单克隆与多克隆双抗体夹心法测定血清白介素- 2 (IL - 2)及可溶性白介素- 2受体(SIL - 2r)水平。

Methods: Monoclonal and poly - clonal dual antibody sandwich method was used to assay the levels of serum interleukin - 2 (IL - 2) and soluble interleukin - 2 receptor (SIL - 2r).

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采用多克隆双抗体夹心elisa法快速检测狂犬病毒抗原含量。

The rabies virus antigen contents were detected by double antibody sandwich ELISA assay using polyclonal antibody.

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