...mphatic metastasis eal-time quantitative RT-PCR,real-time qRT-PCR)技术、免疫组织化学技术以及流式细胞技术(flow cytometry,FCM)检测趋化因子受体CCR6,CCR7及其配体(CCL20,CCL19/CCL21),以及CD4+ CD..
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...tic metastasis eal-time quantitative RT-PCR,real-time qRT-PCR)技术、免疫组织化学技术以及流式细胞技术(flow cytometry,FCM)检测趋化因子受体CCR6,CCR7及其配体(CCL20,CCL19/CCL21),以及CD4+ CD..
基于2个网页-相关网页
方法利用盐酸利多卡因注射液联合震荡培养法分离纯化胎大鼠脑内小胶质细胞，免疫荧光技术及流式细胞技术鉴定小胶质细胞的纯度。
Method: Fetal rat microglia in brain was isolated and purified by lidocaine hydrochloride with shock culturing. The purification of microglia was detected by immunofluorescence and flow cytometry.
采用双标记流式细胞分析技术，分别检测各组小鼠脾脏及肠系膜淋巴结（MLN）CD80 M和CD86 M的表达。
The number of CD80/CD86 M of spleen and mesenteric lymph node(MLN) were determined by double-label FCM method.
方法采用脂质体介导的质粒转染、流式细胞仪分析、逆转录- PCR及高效液相色谱(HPL C)分析等实验技术方法做有关分析研究。
Methods The plasmid transfection mediated by liposomal transfection reagent, HPLC analysis and reverse transcription-PCR analysis were used in this study.
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