本文还详细研究了菌龄、酶系统、酶解温度和时间等多种因素对这两种鸡纵菌原生质体形成和再生的影响。

While the effects of hyphal age, lytic enzyme system, osmotic stabilizer, enzymolysis temperature and time on the preparation and regeneration of protoplast were also discussed in this paper.

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在原生质体形成及再生的最佳条件下制备原生质体，结果发现，SD - 142菌的原生质体形成率及再生率分别达到了86.8%和31.3%。

Under the optimum conditions, the rates of formation and regeneration of protoplasts of stain SD-142 had reached to 86.8% and 31.3% respectively.

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稳定剂为琥珀酸钠，再生培养方法为平板双层法。 原生质体形成率和再生率分别达到93.8%和37.5%。

The rates of formation and regeneration of protoplasts of strain S23 had reached to 93.8% and 37.5% respectively.

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