采用麸皮再生培养基替代R2YE再生培养基，原生质体再生率、生长及筛选效果得到明显改善。
The effect of protoplast regeneration and screen was enhanced when R2YE medium was replaced by bran medium.
在原生质体形成及再生的最佳条件下制备原生质体，结果发现，SD - 142菌的原生质体形成率及再生率分别达到了86.8%和31.3%。
Under the optimum conditions, the rates of formation and regeneration of protoplasts of stain SD-142 had reached to 86.8% and 31.3% respectively.
稳定剂为琥珀酸钠，再生培养方法为平板双层法。 原生质体形成率和再生率分别达到93.8%和37.5%。
The rates of formation and regeneration of protoplasts of strain S23 had reached to 93.8% and 37.5% respectively.
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