目的构建针对APRIL的免疫抑制分子,并对其原核表达蛋白进行纯化及活性筛选。
Objective To construct, express, purify and screen immunosuppressive molecule against human soluble APRIL (a proliferation-inducing ligand).
结论:原核表达载体的构建、重组蛋白的表达、纯化及多克隆抗体的制备为今后研究该蛋白的功能提供了良好的基础。
CONCLUSION: Recombined prokaryotic expression vector, the purified protein and prepared polyclonal antibody were the necessary materials for further study of this protein.
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