目的改构高致病性禽流感病毒血凝素基因，建立有效的原核表达体系。
Objective To modify the HA1 gene of high pathogenic avian influenza virus (HPAIV) and to build an efficient prokaryotic expression system of it.
利用序列分析技术克隆与抗逆相关的基因，并通过原核表达体系和转基因技术进行功能验证。
The function of gene has been identified using Prokaryotic expression system and Arabidopsis thaliana (A. thaliana) transformation systems. The study has got following results:1.
结论利用原核表达体系成功地表达了不同分子质量的颗粒溶素融合蛋白，为颗粒溶素的后续研究奠定了基础。
Conclusion Different molecular weight granulysin were successfully expressed using prokaryotic expression system, which would be helpful for the further study of granulysin.
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