采用单链聚合酶链反应(PCR)和测序法检测肿瘤组织中VHL基因的突变情况。
Mutation of VHL gene from tumor tissue was detected from tumor tissue by polymerase chain reaction (PCR) and direct sequencing.
方法：聚合酶链反应（PCR）、单链构象多态性（SSCP）分析。
Methods:Polymerase chain reaction (PCR) and single strand conformation polymorphism(SSCP) was used.
方法采用单管四重聚合酶链反应(PCR)技术。
Methods Single barrel four-seat PCR technique was performed.
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