方法应用单因素设计和反应曲面设计(rsd)进行RAPD反应体系的优化。
Methods The optimum reaction systems of RAPD were obtained by single factor design and response surface design (RSD).
结果：单因素设计、正交设计、均匀设计、因子设计是目前微球工艺中常用的优化方法。
RESULTS: Single factor design, orthogonal design, uniform design and factorial design are widely used for the optimization purpose.
方法：试验采用单因素随机区组设计，定株定期进行调查测定，收获后测各器官干重、产量和主要有效成分含量。
Method: Single factor randomized block design was applied, plant samples were collected and investigated periodically, and dry weight, production and the main active ingredient content were measured.
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