这个综述讨论了一些单分子测序技术，它们被期望将在今后的几年间成为可用，并解释了它们将如何影响基因组学研究。

This review discusses several single-molecule sequencing technologies that are expected to become available during the next few years and explains how they might impact on genomics research.

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基于在DNA单分子定位切割和拾取方面的实验进展，提出了一种基于原子力显微镜纳米操纵技术的单分子有序化测序策略。

Thus an ordered sequencing strategy is proposed based on the experimental advancements in positioning dissection and isolation of single DNA molecules with AFM nanomanipulation.

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