...转染 35-36 3.2.5 半定量逆转录-聚合酶反应（semiquantitative-reverse transcription-polymerase chain reaction, SQ-RT-PCR）检测EGFR的表达 36-39 3.2.6 免疫荧光 39 3.2.7 MTT法(噻唑兰比色..
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半定量逆转录PCR semi-quantify RT-PCR
半定量逆转录-pcr semi quantitative reverse transcription polymerase chain reaction
采用半定量逆转录多聚酶链反应(RT - PCR)检测局部内皮素系统ET - 1、ETAR、ETBR及ECE的基因表达。
The gene expression of ET-1, ETAR, ETBR and ECE was evaluated by semi-quantitative reverse transcription polymerase chain response (RT-PCR).
采用免疫组化方法、半定量逆转录聚合酶链反应（RT-PCR）和免疫印迹分析法检测COX-2、CDKN2A在组织中的表达。
The expression of COX-2 and CDKN2A was determined by immunohistochemistry, semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
方法采用半定量pcr方法检测了我国四种小型猪内源性逆转录病毒基因的拷贝数。
Methods Semi-quantitative PCR method was used in this research to estimate PERV copy-number of four mini-pigs in China.
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