免疫组织化学方法结合图像分析检测各组脊神经节sp的阳性神经元数和平均光密度，并计算阳性细胞百分率。

Immunohistochemical staining and image analysis were used to detect the number of the positive neurons and the mean optical density and the percentages of SP positive cells in the DRG.

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应用免疫细胞化学方法检测细胞凋亡光密度值情况。

Immunocytochemical method to detect apoptosis situation by optical density.

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