前言： 目的：用组织化学和动物实验方法观察盐酸四环素根管给药后局部组织的反应，探讨其作为根管治疗术用药的前景。
AIM:To observe the reaction of the topical tissue by using tetracycline-HCI as a root canal treatment and discuss the future of tetracycline in that treatment.
利用免疫组织化学方法及图像分析系统对各组动物脊髓内星形胶质细胞的时空分布和变化进行观察和分析。
Immunohistochemical technique and imaging analysis technique were used to detect the temporal and spatial distributions and changes of the astrocyte in spinal cord in the animals of each group.
动物接种实验证实该细胞可在正常小鼠皮下成瘤，免疫组织化学检测可见该细胞系在C57BL/6小鼠皮下接种胰腺癌模型中可表达MUC1。
Then in the animal model, it could form tumors in the normal C57BL/6 mice and immunohistological examination showed that this cell line could express MUC1 in vivo.
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