...中分离到的一株内生真菌EN-22的次生代谢产物的化学成分进行了研究。利用正相硅胶柱层析、葡聚糖凝胶Sephadex LH-20柱层析、制备薄层层析（PTLC）以及重结晶等分离手段从该菌发酵液中分离得到15个化合物，通过一维、二维核磁共振技术、质谱技术等鉴定了所有化合物的结构，分别...
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采用硅胶、聚酰胺、葡聚糖凝胶LH-20柱层析法和制备薄层层析法对乙酸乙酯和正丁醇提取物进行了分离、纯化。
The ethyl acetate and n-butanol extraction were chromatographed on silica gel column, polyamide column, sephadex LH-20 column and preparing TLC, respectively.
方法：用升华法制备样品，用薄层层析法分离，用光谱法鉴定。
Methods: The samples were prepared with method of sublimation, separated with thin layer chromatography and identified with spectrometry.
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