本实验采用结扎大鼠的左冠状动脉前降支制备急性心肌缺血(Acute myocardial ischemia，AMI)的经典模型。研究槐定堿对大鼠心肌梗死范围的影响，从而探讨槐定堿对大鼠急性心肌缺血损伤的保护作用。
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方法：结扎大鼠左冠状动脉前降支制备急性心肌缺血模型。
Method: The left anterior descending coronary arteries of rats were ligated.
②对异丙肾上腺素所致急性心肌缺血小鼠缺氧存活时间的影响：采用异丙肾上腺素皮下注射制备小鼠急性心肌缺血模型，观察各组小鼠常压耐缺氧存活时间。
The model of mice with acute myocardial ischemia was made by subcutaneous injection of Iso in order to observe the survival time in normobaric hypoxia of mice in each group.
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