将胰岛素原基因融合到金色葡萄球菌蛋白a的基因上，构建成大肠杆菌中基因融合的外分泌表达载体。
The secretion expression vector of fusion gene in E. coli has constructed by fussing the proinsulin gene to the gene of staphylococcal protein a.
外源淀粉水解酶能在酵母中高效表达并分泌，其主要因素在于：在构建载体时组入强启动子；
The main factors for high efficiency expression and secretion of exogenous amylum hydrolase in S. cerevisiae are as follows: involvement of strong promoter in carrier construction;
结论构建了人瘦素的哺乳动物细胞表达载体，并成功地在COS 7细胞中获得重组人瘦素的分泌表达。
Conclusion Cloning and expression recombinants of human leptin are obtained and secretive recombinant human leptin is successfully expressed in COS-7 cells.
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