将胰岛素原基因融合到金色葡萄球菌蛋白a的基因上,构建成大肠杆菌中基因融合的外分泌表达载体。
The secretion expression vector of fusion gene in E. coli has constructed by fussing the proinsulin gene to the gene of staphylococcal protein a.
外源淀粉水解酶能在酵母中高效表达并分泌,其主要因素在于:在构建载体时组入强启动子;
The main factors for high efficiency expression and secretion of exogenous amylum hydrolase in S. cerevisiae are as follows: involvement of strong promoter in carrier construction;
结论构建了人瘦素的哺乳动物细胞表达载体,并成功地在COS 7细胞中获得重组人瘦素的分泌表达。
Conclusion Cloning and expression recombinants of human leptin are obtained and secretive recombinant human leptin is successfully expressed in COS-7 cells.
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